| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Heartland FC HFC Stars |
05085-0011B4U-0003 | Phoukhong Manivong |
| A2 | Dreamers FC DFC MPA NPL 08B |
03134-0011B3U-1766 | Kenan Sahuric |
| A3 | z-Developmental Players Academy DPA Bhoys 07B |
0N076-0011B4U-0003 | Kade Hollendieck |
| A4 | Schuyler Youth Soccer Warriors 2007/2008 |
04018-0012B4U-0019 | Mynor Hernandez |
| B1 | Gretna Elite Academy GEA Gold 07B |
02077-0011B4U-0034 | Adi Sakic |
| B2 | z-Infinite Sports World ISW Blaze |
03124-0012B4U-0004 | Jeff Oksman |
| B3 | Columbus Soccer Club CSC Chaos 2007 |
04017-0012B4U-0143 | John Bailey |
| B4 | Fremont Soccer Club FSC United Gold 07/08 |
04054-0013B4U-0139 | Jose Lango |